Anti-Ki67 antibody [EPR3610]
| Name | Anti-Ki67 antibody [EPR3610] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92742 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3610 |
| Applications | IHC-F WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues in Human Ki67 |
| Description | Rabbit Monoclonal |
| Gene | MKI67 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing Ramos cells stained with ab92742 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92742, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab92742 showing positive staining in Colonic adenocarcinoma tissue.
ab92742 showing positive staining in Normal colon tissue.
ab92742, at a 1/500 dilution, staining Ki67 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
ab92742, at a 1/500 dilution, staining Ki67 in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.
ab92742, at a 1/250 dilution, staining Ki67 in HeLa cells by Immunofluorescence.
ab92742 showing positive staining in Ovarian carcinoma tissue.
ab92742 showing positive staining in Cervical carcinoma tissue.
ab92742 staining Ki67 in human tonsil tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized and blocked with 4% serum + 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 18 hours at 4°C. An Alexa Fluor® 568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Magnification: 20X. Counterstained with DAPI.See Abreview
![Anti-Ki67 antibody [EPR3610] (ab92742) at 1/500 dilution + HeLa lysates at 10 µgSecondaryGoat anti-Rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/12508_Ki67-Primary-antibodies-ab92742-1.jpg)
Anti-Ki67 antibody [EPR3610] (ab92742) at 1/500 dilution + HeLa lysates at 10 µgSecondaryGoat anti-Rabbit HRP at 1/2000 dilution
Product References
Clinicopathological significance of non-small cell lung cancer with high - Clinicopathological significance of non-small cell lung cancer with high
Chen Z, Wang T, Cai L, Su C, Zhong B, Lei Y, Xiang AP. J Exp Clin Cancer Res. 2012 Feb 2;31:10.
Tetrastatin, the NC1 domain of the alpha4(IV) collagen chain: a novel potent - Tetrastatin, the NC1 domain of the alpha4(IV) collagen chain: a novel potent
Brassart-Pasco S, Senechal K, Thevenard J, Ramont L, Devy J, Di Stefano L, Dupont-Deshorgue A, Brezillon S, Feru J, Jazeron JF, Diebold MD, Ricard-Blum S, Maquart FX, Monboisse JC. PLoS One. 2012;7(4):e29587.