Anti-KIF14 antibody
| Name | Anti-KIF14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3746 |
| Prices | $370.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape |
| Antigen | Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14)encoded within exon 2 |
| Description | Rabbit Polyclonal |
| Gene | KIF14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Sample: Hela whole cell lysate1) WB - 50µg.2) IP - 1mg. Antibody: Affinity purified Rabbit anti-KIF (ab3746)1) & 2) WB - 1 / 4000 dilution.2) IP - concentration of 3µg / mg of lysate. Detection: ECL.
ICC/IF image of ab3746 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.