Anti-KIN antibody (ab22842) at 1 µg/ml + HepG2 cell lysate with 5% skim milk / PBS bufferSecondaryHRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,025.Performed under reducing conditions.
Paraffin embeded tissue: Human kidney, Cells with positive label (indicated with arrows): Epithelial cells of renal tubule, Antibody concentration: 4-8 µg/ml, Magnification: x400
Paraffin embeded tissue: Human spermatophore, Cells with positive label (indicated with arrows): Epithelial cells, Antibody concentration: 4-8 µg/ml, Magnification: x400