Anti-Kisspeptin antibody
| Name | Anti-Kisspeptin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab80994 |
| Prices | $379.00 |
| Sizes | 500 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P IHC-F |
| Species Reactivities | Human |
| Antigen | Synthetic peptide from the N terminal region of human Kisspeptin conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | KISS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab80994 stained JEG-3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80994, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Kisspeptin regulation of genes involved in cell invasion and angiogenesis in - Kisspeptin regulation of genes involved in cell invasion and angiogenesis in
Francis VA, Abera AB, Matjila M, Millar RP, Katz AA. PLoS One. 2014 Jun 12;9(6):e99680.
Altered placental expression of kisspeptin and its receptor in pre-eclampsia. - Altered placental expression of kisspeptin and its receptor in pre-eclampsia.
Cartwright JE, Williams PJ. J Endocrinol. 2012 Jul;214(1):79-85.