Anti-KLF15 antibody [2G8] - ChIP Grade
| Name | Anti-KLF15 antibody [2G8] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab81604 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2G8 |
| Applications | ChIP WB ELISA FC EMSA |
| Species Reactivities | Human |
| Antigen | Purified recombinant fragment of human KLF15 expressed in E |
| Description | Mouse Monoclonal |
| Gene | KLF15 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-KLF15 antibody [2G8] - ChIP Grade (ab81604) at 1/500 dilution + HepG2 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13367_KLF15-Primary-antibodies-ab81604-1.jpg)
Anti-KLF15 antibody [2G8] - ChIP Grade (ab81604) at 1/500 dilution + HepG2 cell lysate
![Overlay histogram showing HepG2 cells stained with ab81604 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81604, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13368_KLF15-Primary-antibodies-ab81604-2.jpg)
Overlay histogram showing HepG2 cells stained with ab81604 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81604, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
The glucocorticoid receptor and KLF15 regulate gene expression dynamics and - The glucocorticoid receptor and KLF15 regulate gene expression dynamics and
Sasse SK, Mailloux CM, Barczak AJ, Wang Q, Altonsy MO, Jain MK, Haldar SM, Gerber AN. Mol Cell Biol. 2013 Jun;33(11):2104-15.
Molecular cloning, characterization, genomic organization and promoter analysis - Molecular cloning, characterization, genomic organization and promoter analysis
Teylaert B, Meurice E, Bobowski M, Harduin-Lepers A, Gaucher C, Fontayne A, Jorieux S, Delannoy P. BMC Biotechnol. 2011 Jan 5;11:1.
The Kruppel-like factor 15 as a molecular link between myogenic factors and a - The Kruppel-like factor 15 as a molecular link between myogenic factors and a
Dmitriev P, Petrov A, Ansseau E, Stankevicins L, Charron S, Kim E, Bos TJ, Robert T, Turki A, Coppee F, Belayew A, Lazar V, Carnac G, Laoudj D, Lipinski M, Vassetzky YS. J Biol Chem. 2011 Dec 30;286(52):44620-31.