Anti-KLF15 antibody [EPR14131] - N-terminal

• Supplier: Abcam
• Catalog: ab185958
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14131
• Applications: WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLF15 aa 1-100 (N terminal)
• Blocking Peptide: KLF15 peptide
• Description: Rabbit Monoclonal
• Gene: KLF15
• Conjugate: Unconjugated

Product images

All lanes : Anti-KLF15 antibody [EPR14131] - N-terminal (ab185958) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-KLF15 antibody [EPR14131] - N-terminal (ab185958) at 1/1000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : PC12 cell lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-KLF15 antibody [EPR14131] - N-terminal (ab185958) at 1/2000 dilutionLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate with immunizing peptideLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescence analysis of 4% paraformaldehyde fixed HEK293 cells, labeling KLF15 (red) with ab185958 at 1/500 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of 4% paraformaldehyde fixed HepG2 cells, labeling KLF15 (red) with ab185958 at 1/500 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Flow cytometry analysis of KLF15 expression in HepG2 cells using ab185958 at 1/220 dilution (red) and a rabbit IgG as negative control (green).