![Overlay histogram showing HEK293 cells stained with ab130243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13433_ab130243-5-ab130243FC.jpg)
Overlay histogram showing HEK293 cells stained with ab130243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-KLF4 antibody [1E6] (ab130243) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : KLF4-hIgGFc transfected HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13434_KLF4-Primary-antibodies-ab130243-5.jpg)
All lanes : Anti-KLF4 antibody [1E6] (ab130243) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : KLF4-hIgGFc transfected HEK293 cell lysate
ab130243, at 1/200 dilution, staining KLF4 in paraffin-embedded Human colon cancer tissue by Immunohistochemistry with DAB staining.
ab130243, at 1/200 dilution, staining KLF4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining.
ab130243, at 1/200 dilution, staining KLF4 in ECA109 cells by Immunofluorescence (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.