KLF4

Gene Symbol KLF4
Entrez Gene 9314
Alt Symbol EZF, GKLF
Species Human
Gene Type protein-coding
Description Kruppel-like factor 4 (gut)
Other Description Krueppel-like factor 4|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut-enriched krueppel-like factor
Swissprots B2R8S4 O43474 L0R4N5 Q9UNP3 Q5T3J9 Q5T3J8 L0R3I6 P78338 Q8N717 B3KT79
Accessions ABG25917 CDM21965 EAW59019 EAW59020 EAW59021 O43474 AF022184 AAC03462 AF105036 AAD42165 AK095134 BAG52991 AK095894 AK313489 BAG36271 BC029923 AAH29923 BC030811 AAH30811 BU739190 DN995850 DQ891793 ABM82719 DQ894975 ABM85901 HF546201 CCO02787 HF546202 CCO02788 HF546213 CCO02799 HM026463 ADL14248 U70663 AAB48399 XM_005252305 XP_005252362 NM_004235 NP_004226
Function Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. {ECO:0000269|PubMed:17308127, ECO:0000269|PubMed:20071344}.
Subcellular Location Nucleus.
Top Pathways Signaling pathways regulating pluripotency of stem cells

KLF4 Antibody - 4038 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using KLF4 antibody.

KLF4 (D1F2) Rabbit mAb - 12173 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1F2
Western blot analysis of extracts from various cell lines using KLF4 (D1F2) Rabbit mAb.

StemLight™ iPS Cell Reprogramming Antibody Kit - 9092 from Cell Signaling Technology

Prices $409.00
Sizes 1 kit (6 x 20 µl)
Clonality Polyclonal
Confocal immunofluorescent analysis of iPS cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP ® Rabbit mAb #3579, Nanog (D73G4) XP ® Rabbit mAb #4903, c-Myc (D84C12) XP ® Rabbit mAb #5605, and LIN28A (D84C11) XP ® Rabbit mAb #3695 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

GKLF (B-8) - sc-393462 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GKLF (B-8): sc-393462. Western blot analysis of GKLF expression in non-transfected 293T: sc-117752 (A), mouse GKLF transfected 293T: sc-125385 (B) and NIH/3T3 (C) whole cell lysates.

GKLF (H-180) - sc-20691 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GKLF (H-180): sc-20691. Western blot analysis of GKLF expression in HeLa nuclear extract.

GKLF (B-9) - sc-166100 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GKLF (B-9): sc-166100. Western blot analysis of GKLF expression in non-transfected: sc-117752 (A) and human GKLF transfected: sc-114641 (B) 293T whole cell lysates.

GKLF (T-16) - sc-12538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GKLF (T-16): sc-12538. Western blot analysis of GKLF expression in HeLa (A) and A-431 (B) nuclear extracts.

GKLF (C-6) - sc-166101 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GKLF (C-6): sc-166101. Western blot analysis of GKLF expression in non-transfected: sc-117752 (A) and human GKLF transfected: sc-114641 (B) 293T whole cell lysates.

GKLF (A-2) - sc-166190 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GKLF (A-2): sc-166190. Western blot analysis of GKLF expression in non-transfected: sc-117752 (A) and human GKLF transfected: sc-114641 (B) 293T whole cell lysates.

GKLF (B-6) - sc-166229 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GKLF (B-6): sc-166229. Western blot analysis of GKLF expression in non-transfected: sc-117752 (A) and human GKLF transfected: sc-114641 (B) 293T whole cell lysates.

GKLF (F-8) - sc-166238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GKLF (F-8): sc-166238. Western blot analysis of GKLF expression in non-transfected: sc-117752 (A) and human GKLF transfected: sc-114641 (B) 293T whole cell lysates.

Anti-KLF4 antibody - ab106629 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-KLF4 antibody (ab106629) at 1 µg/mlLane 2 : Anti-KLF4 antibody (ab106629) at 2 µg/mlLane 1 : Human liver tissue lysateLane 2 : Human liver tissue lysateLysates/proteins at 15 µg per lane.

Anti-KLF4 antibody - ab155808 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-KLF4 antibody (ab155808) at 1/500 dilution + HeLa whole cell lysate at 30 µg

Anti-KLF4 antibody - ab77090 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-KLF4 antibody (ab77090) at 0.1 µg/ml + Human duodenum lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-KLF4 antibody - ab72543 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : Anti-KLF4 antibody (ab72543) at 1 µg/mlLanes 3 - 4 : Anti-KLF4 antibody (ab72543) at 2 µg/mlLane 1 : 293 cell lysate Lane 2 : 293 cell lysate with immunizing peptideLane 3 : 3T3 cell lysate Lane 4 : RAW cell lysate

Anti-KLF4 antibody [ EPR3550(2)(ABC)] - ab151733 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR3550(2)(ABC)
All lanes : Anti-KLF4 antibody [ EPR3550(2)(ABC)] (ab151733) at 1/10000 dilution (purified)Lane 1 : HeLa cell lysateLane 2 : NCCIT cell lysateLane 3 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-KLF4 antibody [1E5] - ab104846 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E5
All lanes : Anti-KLF4 antibody [1E5] (ab104846) at 1/500 dilutionLane 1 : KLF4 (aa2-470)-hIgGFc transfected HEK293 Lane 2 : HEK293 cell lysate

Anti-KLF4 antibody [1E6] - ab130243 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E6
Overlay histogram showing HEK293 cells stained with ab130243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-KLF4 antibody [56CT5.1.6] - ab75486 from Abcam

Prices $394.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 56CT5.1.6
Anti-KLF4 antibody [56CT5.1.6] (ab75486) at 1/200 dilution + KLF4 recombinant protein

Anti-KLF4 antibody [AT4E6] - ab118961 from Abcam

Host Mouse
Clonality Monoclonal
Clone AT4E6
ab118961 at 20&microg/ml staining KLF4 in Human small intestine tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-KLF4 antibody [EPR3550(2)(ABC)] (Alexa Fluor® 488) - ab193246 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR3550(2)(ABC)
ab193246 staining KLF4 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab193246 at a working dilution of 1 in 50 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

Anti-KLF4 antibody [EPR3550(2)(ABC)] (Alexa Fluor® 555) - ab201730 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3550(2)(ABC)
ab201730 staining KLF4 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201730 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-KLF4 antibody [EPR3550(2)(ABC)] (Alexa Fluor® 568) - ab201731 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3550(2)(ABC)
ab201731 staining KLF4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201731 at a 1/100 dilution (green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-KLF4 antibody [EPR3550(2)(ABC)] (Alexa Fluor® 594) - ab201729 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3550(2)(ABC)
ab201729 staining KLF4 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201729 at a 1/50 dilution (red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Anti-KLF4 antibody [EPR3550(2)(ABC)] (Alexa Fluor® 647) - ab192984 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3550(2)(ABC)
ab192984 staining KLF4 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192984 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 488 Goat anti-mouse IgG (H&L - preadsorbed) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).
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