Western blot analysis of extracts from A-431 (positive) and MCF7 (negative) cells using P-Cadherin (12H6) Mouse mAb (upper) and Pan-Cadherin (28E12) Rabbit mAb #4073 (lower).
Immunoprecipitation of P-cadherin from A-431 cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or P-Cadherin (12H6) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using P-Cadherin (12H6) Mouse mAb.
Confocal immunofluorescent analysis of A-431 (positive, left) and MCF7 (negative, right) cells using P-Cadherin (12H6) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of MCF7 cells (blue) and A-431 cells (green) using P-Cadherin (12H6) Mouse mAb. Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4408 was used as secondary antibody.