All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (purified)Lane 1 : Human fetal kidneyLane 2 : Human testisLane 3 : Human fetal thymusLysates/proteins at 20 µg per lane.SecondaryHRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
All lanes : Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution (unpurified)Lane 1 : Human fetal kidney lysatesLane 2 : Human testis lysatesLane 3 : Human fetal thymus lysatesLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.