Anti-KMT1E / SETDB1 antibody [5H6A12]
| Name | Anti-KMT1E / SETDB1 antibody [5H6A12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab107225 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 5H6A12 |
| Applications | FC WB ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Human, Monkey |
| Antigen | Purified recombinant fragment of human SETDB1 expressed in E |
| Description | Mouse Monoclonal |
| Gene | SETDB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilutionLane 1 : MCF-7Lane 2 : T47D Lane 3 : HEK293Lane 4 : JURKAT Lane 5 : NIH/3T3Lane 6 : F9Lane 7 : RAW246.7Lane 8 : COS-7](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13745_KMT1E-SETDB1-Primary-antibodies-ab107225-1.jpg)
All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilutionLane 1 : MCF-7Lane 2 : T47D Lane 3 : HEK293Lane 4 : JURKAT Lane 5 : NIH/3T3Lane 6 : F9Lane 7 : RAW246.7Lane 8 : COS-7
Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).
![Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13747_KMT1E-SETDB1-Primary-antibodies-ab107225-3.jpg)
Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.