SETDB1

Gene Symbol SETDB1
Entrez Gene 9869
Alt Symbol ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21
Species Human
Gene Type protein-coding
Description SET domain, bifurcated 1
Other Description ERG-associated protein with SET domain|ERG-associated protein with a SET domain, ESET|H3-K9-HMTase 4|histone H3-K9 methyltransferase 4|histone-lysine N-methyltransferase SETDB1|histone-lysine N-methyltransferase, H3lysine-9 specific 4|lysine N-methyltransferase 1E|tudor domain containing 21
Swissprots Q96GM9 Q5SZE0 A6NEW2 Q5SZD9 Q15047 Q5SZE7 Q5SZD8
Accessions CBJ06473 CBJ06474 EAW53503 EAW53504 EAW53505 EAW53506 EAW53507 EAW53508 Q15047 AI582266 AK095075 AK302461 BAG63752 AK308335 BC009362 AAH09362 BC028671 AAH28671 D31891 BAA06689 DA418593 DB062762 XM_005245641 XP_005245698 NM_001145415 NP_001138887 NM_001243491 NP_001230420 NM_012432 NP_036564
Function Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. {ECO:0000269|PubMed:12869583, ECO:0000269|PubMed:14536086, ECO:0000269|PubMed:15327775, ECO:0000269|PubMed:17952062}.
Subcellular Location Nucleus. Chromosome. Note=Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Tissue Specificity Widely expressed. High expression in testis.
Top Pathways Signaling pathways regulating pluripotency of stem cells, Lysine degradation

ESET (C1C12) Rabbit mAb - 2196 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C1C12
Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb.

ESET (A-7) - sc-271553 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ESET (A-7): sc-271553. Western blot analysis of ESET expression in F9 whole cell lysate.

ESET (G-4) - sc-271488 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ESET (G-4): sc-271488. Western blot analysis of ESET expression in F9 whole cell lysate.

ESET (A-1) - sc-166621 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ESET (A-1): sc-166621. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization.

ESET (S-14) - sc-46112 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ESET (H-300) - sc-66884 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ESET (H-300): sc-66884. Western blot analysis of ESET expression in F9 whole cell lysate.

ESET (FA-9) - sc-130437 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
ESET (FA-9): sc-130437. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-KMT1E / SETDB1 antibody - ab61329 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-KMT1E / SETDB1 antibody (ab61329) at 1 µg/ml + SW13 whole cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-KMT1E / SETDB1 antibody - ab5430 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KMT1E / SETDB1 antibody - ab115340 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115340 at 5ug/ml staining KMT1E/SETDB1 in Formalin-fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-KMT1E / SETDB1 antibody [5H6A12] - ab107225 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H6A12
All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilutionLane 1 : MCF-7Lane 2 : T47D Lane 3 : HEK293Lane 4 : JURKAT Lane 5 : NIH/3T3Lane 6 : F9Lane 7 : RAW246.7Lane 8 : COS-7

Anti-KMT1E / SETDB1 antibody - ChIP Grade - ab12317 from Abcam

Prices $392.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Whole cell lysate (2 mg) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4). Rabbit anti-SETDB1 antibodies; ab12317 (2 and 4) and a competitor's antiserum (1 and 3) were used at 1 mcg/mg lysate for IP. ab12317 was used at 0.2 mcg/ml for Western Blot. Detection: Chemiluminescence with exposure times of 1 to 5 minutes.

Anti-KMT1E / SETDB1 antibody - N-terminal - ab71194 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KMT1E / SETDB1 antibody - N-terminal (ab71194) at 1/500 dilutionLane 1 : Cell lysates prepared from non trasfected 293 cells Lane 2 : Cell lysates prepared from 293 cells transfected with SETDB1 gene

Anti-ESET / SETDB1 Antibody (aa475-489) - LS-C47411 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (aa2-101, clone 10B2474, Azide-free) - LS-C183714 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10B2474

Anti-ESET / SETDB1 Antibody (C-Terminus, Biotin) - LS-C282645 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (C-Terminus, AP) - LS-C282647 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (C-Terminus, APC) - LS-C282646 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (C-Terminus, Azide-free, HRP) - LS-C282650 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (C-Terminus, PE) - LS-C282651 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (C-Terminus, FITC) - LS-C282648 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (aa1125-1175) - LS-C287050 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (clone 5H6D4) - LS-C169281 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H6D4
Western blot using SETDB1 mouse monoclonal antibody against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate.

Anti-ESET / SETDB1 Antibody (C-Terminus) - LS-C328637 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ESET / SETDB1 Antibody (N-Terminus) - LS-C201353 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HT1080 Whole cell . SETDB1 antibody dilution 1.0 ug/ml.
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