All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab69836) at 1.25 µg/mlLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysateLane 3 : HepG2 cell lysateLane 4 : Molecular weight markerSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Immunohistochemical analysis of paraffin-embedded human placenta using ab69836 at 4 µg/ml. Positively labelled trophoblast cells are indicated with arrows.
ICC/IF image of ab69836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.