Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86]
| Name | Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3798 |
| Prices | $401.00 |
| Sizes | 400 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 43AT551.86 |
| Applications | ICC/IF ICC/IF WB IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Fusion protein (GST-tag) corresponding to Human KMT5A/ SETD8/ Pr-SET7 (N terminal) |
| Description | Mouse Monoclonal |
| Gene | SETD8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798) at 1/1000 dilutionLane 1 : SAOS-2 (human epithelial-like osteosarcoma cell line) whole cell lysatesLane 2 : NIH/3T3 whole cell lysateLysates/proteins at 35 µg per lane.SecondaryPeroxidase-conjugated goat anti-mouse IgG (H+L) at 1/5000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13808_ab3798_4.jpg)
All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798) at 1/1000 dilutionLane 1 : SAOS-2 (human epithelial-like osteosarcoma cell line) whole cell lysatesLane 2 : NIH/3T3 whole cell lysateLysates/proteins at 35 µg per lane.SecondaryPeroxidase-conjugated goat anti-mouse IgG (H+L) at 1/5000 dilution
Immunoflouresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab3798 used: 1/10. Green = ab3798 detected with secondary antibody (Alexa 488). Blue = DAPI stain.
ab3798 (1µg/ml) staining SETD8 in Human thyroid gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of epithelial cells of the thyroid follicle.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
![Overlay histogram showing HeLa cells stained with ab3798 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3798, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13811_KMT5A-SETD8-Pr-SET7-Primary-antibodies-ab3798-2.jpg)
Overlay histogram showing HeLa cells stained with ab3798 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3798, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
![All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : 293T cell lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG (H+L) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13812_ab3798-239077-ab3798wb.jpg)
All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : 293T cell lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG (H+L) at 1/3000 dilution
Product References
Establishing a hematopoietic genetic network through locus-specific integration - Establishing a hematopoietic genetic network through locus-specific integration
DeVilbiss AW, Boyer ME, Bresnick EH. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3398-407. doi:
Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating - Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating
Takawa M, Cho HS, Hayami S, Toyokawa G, Kogure M, Yamane Y, Iwai Y, Maejima K, Ueda K, Masuda A, Dohmae N, Field HI, Tsunoda T, Kobayashi T, Akasu T, Sugiyama M, Ohnuma S, Atomi Y, Ponder BA, Nakamura Y, Hamamoto R. Cancer Res. 2012 Jul 1;72(13):3217-27.
A novel tumor suppressor network in squamous malignancies. - A novel tumor suppressor network in squamous malignancies.
Costa C, Santos M, Segrelles C, Duenas M, Lara MF, Agirre X, Prosper F, Garcia-Escudero R, Paramio JM. Sci Rep. 2012;2:828.
Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target - Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target
Li Z, Nie F, Wang S, Li L. Proc Natl Acad Sci U S A. 2011 Feb 22;108(8):3116-23. doi:
Dual role for the methyltransferase G9a in the maintenance of beta-globin gene - Dual role for the methyltransferase G9a in the maintenance of beta-globin gene
Chaturvedi CP, Hosey AM, Palii C, Perez-Iratxeta C, Nakatani Y, Ranish JA, Dilworth FJ, Brand M. Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18303-8. doi:
Direct interaction between SET8 and proliferating cell nuclear antigen couples - Direct interaction between SET8 and proliferating cell nuclear antigen couples
Huen MS, Sy SM, van Deursen JM, Chen J. J Biol Chem. 2008 Apr 25;283(17):11073-7.