SETD8

Gene Symbol SETD8
Entrez Gene 387893
Alt Symbol KMT5A, PR-Set7, SET07, SET8
Species Human
Gene Type protein-coding
Description SET domain containing (lysine methyltransferase) 8
Other Description H4-K20-HMTase SETD8|H4-K20-specific histone methyltransferase|H4K20-specific histone methyltransferase splice variant Set8b|N-lysine methyltransferase SETD8|PR/SET domain containing protein 8|PR/SET domain-containing protein 07|PR/SET07|SET domain-containing protein 8|histone-lysine N-methyltransferase SETD8|lysine N-methyltransferase 5A
Swissprots Q86W83 Q9NQR1 A8K9D0 Q8TD09
Accessions EAW98406 EAW98407 EAW98408 EAW98409 Q9NQR1 AF287261 AAF97812 AK292645 BAF85334 AY064546 AAL40879 AY102937 AAM47033 BC050346 AAH50346 BQ773996 HQ259683 ADP08984 HQ448034 ADQ32515 XM_006719394 XP_006719457 XM_011538332 XP_011536634 XM_011538333 XP_011536635 XR_944546 NM_020382 NP_065115
Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration. {ECO:0000269|PubMed:12086618, ECO:0000269|PubMed:12121615, ECO:0000269|PubMed:15200950, ECO:0000269|PubMed:15933069, ECO:0000269|PubMed:15933070, ECO:0000269|PubMed:16517599, EC
Subcellular Location Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress- dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
Top Pathways Lysine degradation

SET8 (C18B7) Rabbit mAb - 2996 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C18B7
Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb.

Pr-Set7 (H-300) - sc-135009 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pr-Set7 (H-300): sc-135009. Western blot analysis of Pr-Set7 expression in NIH/3T3 (A), 3611-RF (B), MM-142 (C), RAW 264.7 (D), LADMAC (E) and WEHI-231 (F) nuclear extracts.

Pr-Set7 (A-19) - sc-54995 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pr-Set7 (A-19): sc-54995. Western blot analysis of Pr-Set7 expression in A-431 nuclear extract.

Pr-Set7 (T-18) - sc-55000 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pr-Set7 (S-19) - sc-54999 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-KMT5A / SETD8 / Pr-SET7 antibody - ab111691 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-KMT5A / SETD8 / Pr-SET7 antibody (ab111691) at 1/2000 dilution + U-87 MG whole cell lysate at 30 µg

Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] - ab3798 from Abcam

Prices $401.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 43AT551.86
All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT551.86] (ab3798) at 1/1000 dilutionLane 1 : SAOS-2 (human epithelial-like osteosarcoma cell line) whole cell lysatesLane 2 : NIH/3T3 whole cell lysateLysates/proteins at 35 µg per lane.SecondaryPeroxidase-conjugated goat anti-mouse IgG (H+L) at 1/5000 dilution

Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT890] - ab71024 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 43AT890
Anti-KMT5A / SETD8 / Pr-SET7 antibody [43AT890] (ab71024) at 1/100 dilution + 293 cells lysate at 12.5 µg

Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)] - ab177488 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11321(2)
All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11321(2)] (ab177488) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : Saos-2 cell lysateLane 3 : HCT-116 cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11322] - ab157462 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11322
All lanes : Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR11322] (ab157462) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Saos-2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-KMT5A / SETD8 / Pr-SET7 antibody - C-terminal - ab135493 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-KMT5A / SETD8 / Pr-SET7 antibody - C-terminal (ab135493) at 1/100 dilution + K562 cell line lysate at 12.5 µg

Anti-SETD8 / SET8 Antibody (clone 43AT551.86.76) - LS-C344162 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 43AT551.86.76
Immunohistochemical of paraffin-embedded H. testis section using SET07 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Anti-SETD8 / SET8 Antibody - LS-C126149 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SETD8 / SET8 Antibody (Biotin) - LS-C180980 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SETD8 / SET8 Antibody (DY650) - LS-C180981 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SETD8 / SET8 Antibody (DY488) - LS-C180982 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SETD8 / SET8 Antibody (HRP) - LS-C180983 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SETD8 / SET8 Antibody (N-Terminus, clone 43AT890) - LS-C96455 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 43AT890
The anti-SET07 antibody is used in Western blot to detect SET07 in 293 cells.

Anti-SETD8 / SET8 Antibody (aa362-393) - LS-C98680 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of SET07 Antibody in K562 cell line lysates (35 ug/lane). SET07 (arrow) was detected using the purified antibody.

Anti-SETD8 / SET8 Antibody (DY550) - LS-C180984 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SETD8 / SET8 Antibody (N-Terminus, APC) - LS-C245784 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-SETD8 / SET8 Antibody (N-Terminus, AP) - LS-C245785 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-SETD8 / SET8 Antibody (N-Terminus, Biotin) - LS-C245786 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-SETD8 / SET8 Antibody (N-Terminus, FITC) - LS-C245787 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-SETD8 / SET8 Antibody (N-Terminus, PE) - LS-C245789 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
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