Anti-KRAS antibody
| Name | Anti-KRAS antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55391 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 16-126 of Human cK Ras |
| Description | Mouse Monoclonal |
| Gene | KRAS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab55391 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55391, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/13968_KRAS-Primary-antibodies-ab55391-1.jpg)
Overlay histogram showing HeLa cells stained with ab55391 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55391, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.
ab55391 staining KRAS in Human Gastric Acenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% phosphate buffered formalin, permeabilized with 0.025% of triton X -100 in TBS and blocked with 3% hydrogen peroxide for 5 minutes at room temperature; antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody (1/100 in 0.025% triton X-100-TBS) for 1 hour. An HRP polymer was used for detection.
Product References
Mutant ras elevates dependence on serum lipids and creates a synthetic lethality - Mutant ras elevates dependence on serum lipids and creates a synthetic lethality
Salloum D, Mukhopadhyay S, Tung K, Polonetskaya A, Foster DA. Mol Cancer Ther. 2014 Mar;13(3):733-41.
Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in - Use of synthetic isoprenoids to target protein prenylation and Rho GTPases in
Chen M, Knifley T, Subramanian T, Spielmann HP, O'Connor KL. PLoS One. 2014 Feb 26;9(2):e89892.
Activation of c-Met and upregulation of CD44 expression are associated with the - Activation of c-Met and upregulation of CD44 expression are associated with the
Elliott VA, Rychahou P, Zaytseva YY, Evers BM. PLoS One. 2014 May 13;9(5):e97432.
A polymorphism in a let-7 microRNA binding site of KRAS in women with - A polymorphism in a let-7 microRNA binding site of KRAS in women with
Grechukhina O, Petracco R, Popkhadze S, Massasa E, Paranjape T, Chan E, Flores I, Weidhaas JB, Taylor HS. EMBO Mol Med. 2012 Mar;4(3):206-17.
Ovarian cancer progression is controlled by phenotypic changes in dendritic - Ovarian cancer progression is controlled by phenotypic changes in dendritic
Scarlett UK, Rutkowski MR, Rauwerdink AM, Fields J, Escovar-Fadul X, Baird J, Cubillos-Ruiz JR, Jacobs AC, Gonzalez JL, Weaver J, Fiering S, Conejo-Garcia JR. J Exp Med. 2012 Mar 12;209(3):495-506.
Genomic instability and histone H3 phosphorylation induction by the Ras-mitogen - Genomic instability and histone H3 phosphorylation induction by the Ras-mitogen
Espino PS, Pritchard S, Heng HH, Davie JR. Int J Cancer. 2009 Feb 1;124(3):562-7.