Anti-KRAS antibody - C-terminal (ab172949) at 1/100 dilution + NIH-3T3 cell line lysate at 35 µg
Confocal immunofluorescent analysis of WiDr cells labeling KRAS with ab172949 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) labeling KRAS with ab172949 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.