Anti-Kv4.3 antibody [S75-41]
| Name | Anti-Kv4.3 antibody [S75-41] |
|---|---|
| Supplier | Abcam |
| Catalog | ab99045 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S75-41 |
| Applications | ICC/IF ICC/IF WB IP IHC-P IHC-F FC |
| Species Reactivities | Mouse, Rat, Human, Xenopus |
| Antigen | Fusion protein, corresponding to C terminal amino acids 415-636 of Rat Kv4 |
| Description | Mouse Monoclonal |
| Gene | KCND3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab99045 staining Kv4.3 in Mouse cerebellum tissue sections by Immunohistochemistry.
ab99045 staining Kv4.3 in Mouse cerebellum tissue sections by Immunohistochemistry.
![Anti-Kv4.3 antibody [S75-41] (ab99045) at 1/1000 dilution + Rat brain membrane tissue lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14413_ab99045-199554-ab99045wb.jpg)
Anti-Kv4.3 antibody [S75-41] (ab99045) at 1/1000 dilution + Rat brain membrane tissue lysate
![Overlay histogram showing SH-SY5Y cells stained with ab99045 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14414_Kv43-Primary-antibodies-ab99045-1.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab99045 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab99045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Voltage dependent potassium channel remodeling in murine intestinal smooth muscle - Voltage dependent potassium channel remodeling in murine intestinal smooth muscle
Liu DH, Huang X, Guo X, Meng XM, Wu YS, Lu HL, Zhang CM, Kim YC, Xu WX. PLoS One. 2014 Feb 6;9(2):e86109.
Auxiliary KChIP4a suppresses A-type K+ current through endoplasmic reticulum (ER) - Auxiliary KChIP4a suppresses A-type K+ current through endoplasmic reticulum (ER)
Tang YQ, Liang P, Zhou J, Lu Y, Lei L, Bian X, Wang K. J Biol Chem. 2013 May 24;288(21):14727-41.