Anti-Lamin B1 + B2 antibody [X223]
| Name | Anti-Lamin B1 + B2 antibody [X223] |
|---|---|
| Supplier | Abcam |
| Catalog | ab4825 |
| Prices | $380.00 |
| Sizes | 2 ml |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | X223 |
| Applications | ICC/IF ICC/IF FC WB |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Full length native lamin B2 protein (purified) (Xenopus laevis) (XLKE-A6 cells) |
| Description | Mouse Monoclonal |
| Gene | LMNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab4825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4825, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14806_ab4825-4-ab4825FC.jpg)
Overlay histogram showing HeLa cells stained with ab4825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4825, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Nuclear lamina in COS cells labelled with the Mouse monoclonal to Lamin B1 + B2 (ab4825).Secondary antibody is labelled with Texas Red.
ab4825 at range of dilutions staining human U2 OS cells by Immunocytochemistry/Immunofluorescence. The cells were Paraformaldehyde fixed and incubated with the antibody for 16 hours. Bound antibody was detected using an Alexa- Fluor ® 488 conjugated goat anti-mouse IgG. Of the dilutions tested in the image, 1/5 was found to be optimum.This image is courtesy of an Abreview submitted by Chi Tang on 6 April 2006.See Abreview
Immunofluorescence analysis of B16F10-Nex2 cells, staining Lamin B1 + B2 with ab4825. Cells were treated with 0.3 mM C7H2 for 1, 2, or 3 h at 37 °C. Cells were fixed with 2% formaldehyde before incubating with primary antibody at 1/5 dilution. A biotinylated anti-mouse IgG was used as the secondary antibody and staining was detected using a streptavidin-FITC conjugate.
Product References
beta-Actin-binding complementarity-determining region 2 of variable heavy chain - beta-Actin-binding complementarity-determining region 2 of variable heavy chain
Arruda DC, Santos LC, Melo FM, Pereira FV, Figueiredo CR, Matsuo AL, Mortara RA, Juliano MA, Rodrigues EG, Dobroff AS, Polonelli L, Travassos LR. J Biol Chem. 2012 Apr 27;287(18):14912-22.
Nuclear lamin expression in normal testis and testicular germ cell tumours of - Nuclear lamin expression in normal testis and testicular germ cell tumours of
Machiels BM, Ramaekers FC, Kuijpers HJ, Groenewoud JS, Oosterhuis JW, Looijenga LH. J Pathol. 1997 Jun;182(2):197-204.