LMNB1

Gene Symbol LMNB1
Entrez Gene 4001
Alt Symbol ADLD, LMN, LMN2, LMNB
Species Human
Gene Type protein-coding
Description lamin B1
Other Description lamin-B1
Swissprots B2R6J6 P20700 Q96EI6 Q3SYN7
Accessions AAC37575 EAW48845 EAW48846 P20700 AK303084 BAG64195 AK312603 BAG35493 AU134245 AW512433 BC012295 AAH12295 BC052951 BC078178 AAH78178 BC103723 AAI03724 DQ891592 ABM82518 EU176582 ABW03383 M34458 AAA36162 XR_948250 NM_001198557 NP_001185486 NM_005573 NP_005564
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Subcellular Location Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.

Lamin B1 (D9V6H) Rabbit mAb - 13435 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9V6H
Western blot analysis of extracts from various cell lines and tissues using Lamin B1 (D9V6H) Rabbit mAb.

Lamin B1 (D4Q4Z) Rabbit mAb - 12586 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4Q4Z
Western blot analysis of extracts from various cell lines using Lamin B1 (D4Q4Z) Rabbit mAb.

Lamin B1 Antibody - 9087 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Lamin B (B-10) - sc-374015 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B (B-10): sc-374015. Western blot analysis of Lamin B expression in Y79 (A) and Jurkat (B) whole cell lysates.

Lamin B (C-12) - sc-365214 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B (C-12): sc-365214. Western blot analysis of Lamin B expression in Y79 whole cell lysate.

Lamin B (C-20) - sc-6216 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Lamin B (C-20): sc-6216. Immunofluorescence staining of methanol-fixed F9 cells showing nuclear lamina localization.

Lamin B (C-5) - sc-365962 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B (C-5): sc-365962. Western blot analysis of Lamin B expression in Jurkat (A) and Y79 (B) whole cell lysates.

Lamin B (G-1) - sc-373918 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B (G-1): sc-373918. Western blot analysis of Lamin B expression in Jurkat whole cell lysate.

Lamin B (M-20) - sc-6217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of Lamin B expression in F9 (A), Jurkat (B,D) and Y79 (C) whole cell lysates. Antibodies tested include Lamin B (C-20): sc-6216 (A,B) and Lamin B (M-20): sc-6217 (C,D).

Lamin B1 (A-11) - sc-377000 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B1 (A-11): sc-377000. Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates.

Lamin B1 (E-12) - sc-377001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B1 (E-12): sc-377001. Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates.

Lamin B1 (H-90) - sc-20682 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Lamin B1 (H-90): sc-20682. Immunofluorescence staining of normal mouse lymph node frozen section showing nuclear envelope staining.

Lamin B1 (S-20) - sc-30264 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Lamin B1 (S-20): sc-30264. Immunofluorescence staining of methanol-fixed HeLa cells showing and nuclear envelope localization.

Lamin B1 (ZL-5) - sc-56145 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Lamin B1 (ZL-5): sc-56145. Western blot analysis of Lamin B1 expression in F9 (A), Y79 (B) and MOLT-4 (C) whole cell lysates.

Lamin B1 (119D5-F1) - sc-56143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Lamin B1 (119D5-F1): sc-56143. Western blot analysis of Lamin B1 expression in Jurkat whole cell lysate.

Lamin B1 (8D1) - sc-56144 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lamin B1 (8D1): sc-56144. Western blot analysis of Lamin B1 expression in F9 (A), Y79 (B) and MOLT-4 (C) whole cell lysates.

Anti-Lamin antibody - ab131716 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Lamin B1 + B2 antibody [X223] - ab4825 from Abcam

Prices $380.00
Sizes 2 ml
Host Mouse
Clonality Monoclonal
Clone X223
Overlay histogram showing HeLa cells stained with ab4825 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4825, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Lamin B1 (phospho T575) antibody - ab111278 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Lamin B1 antibody - ab65986 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded mammary cancer tissue staining Lamin B1 in cytoplasm using ab65986 at 1µg/ml. DAB chromogenic reaction.

Anti-Lamin B1 antibody - ab74393 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab74393, at 1/25 dilution, staining Lamin B1 in formalin-fixed, paraffin-embedded human breast carcinoma tissue sections by Immunohistochemistry.

Anti-Lamin B1 antibody - ab22137 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Lamin B1 antibody (ab22137) at 0.5 µg/mlLane 1 : HeLa whole cell lysateLane 2 : Hep-2 whole cell lysateLysates/proteins at 30 µg per lane.Observed band size : 66 kDa (why is the actual band size different from the predicted?)Additional bands at : 35 kDa,62 kDa. We are unsure as to the identity of these extra bands.

Anti-Lamin B1 antibody [4E4] - ab124374 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4E4
ab124374, at 3 µg/ml, staining Lamin B1 in formalin fixed paraffin embedded Human kidney by Immunohistochemistry.

Anti-Lamin B1 antibody [EPR8985(B)] - ab133741 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8985(B)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab133741 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution. 

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (Alexa Fluor® 488) - ab194106 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8985(B)
ab194106 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab94106 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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