Anti-Lamin B1 antibody [EPR8985(B)]

• Supplier: Abcam
• Catalog: ab133741
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8985(B)
• Applications: IP ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Lamin B1 aa 500 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: LMNB1
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling Lamin B1 with ab133741 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/200 dilution (green). Nuclear envelope staining on Ramos cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab133741 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/200 dilution. 

All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/50000 dilution (purified)Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysateLane 2 : Molt-4 (Human lymphoblastic leukemia cell line) cell lysateLane 3 : Y79 (Human retinoblastoma cell line) cell lysateLane 4 : Caco-2 (Human colorectal adenocarcinoma cells) cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/50000 dilution (purified)Lane 1 : C6 (Rat glial tumor cells) cell lysateLane 2 : PC12 (Rat adrenal gland pheochromocytoma) cell lysateLane 3 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLane 4 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/10000 dilution (purified) + Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µgSecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/10000 dilution (unpurified)Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysateLane 2 : Molt4 (Human lymphoblastic leukemia cell line) cell lysateLane 3 : Y79 (Human retinoblastoma cell line) cell lysateLane 4 : Caco 2 (Human colorectal adenocarcinoma cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

All lanes : Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) at 1/10000 dilutionLane 1 : Mouse brain lysatesLane 2 : Mouse heart lysatesLane 3 : Mouse kidney lysatesLane 4 : Mouse spleen lysatesLane 5 : Rat brain lysatesLane 6 : Rat heart lysatesLane 7 : Rat spleen lysatesLane 8 : C6 (Rat glial tumor cells) whole cell lysatesLane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 10 : NIH/3T3 (Mouse embyro fibroblast cells)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of the bladder tissue labeling Lamin B1 with purified ab133741 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemical staining of paraffin embedded Mouse Cerebral cortex with purified ab133741 at a working dilution of 1/300. The secondary antibody used is a HRP polymer for rabbit IgG. Nuclear envelope staining on neuron cells of Cerebral cortex tissue is observed. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lamin B1 with unpurified ab133741 at 1/250.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Lamin B1 with unpurified ab133741 at 1/250.

ab133741 (purified) at 1/20 immunoprecipitating Lamin B1 in Jurkat cells (Lane 1).  For western blotting, ab133741 was used at 1/1000 dilution and an HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

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