Anti-Laminin 5 antibody

• Supplier: Abcam
• Catalog: ab14509
• Prices: $395.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP ICC/IF ICC/IF IHC-F WB ELISA
• Species Reactivities: Mouse, Horse, Dog, Human
• Antigen: Purified Human Laminin 5 protein
• Description: Rabbit Polyclonal
• Gene: LAMA3
• Conjugate: Unconjugated

Product images

ICC/IF image of ab14509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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