ab78286 staining Laminin 5 in Human cultured skin (NHEK, NB1RGB) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Blocking One® for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 10% Blocking One® in PBS) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Rabbit anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
IHC image of Laminin 5 staining in human Normal Colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX