Anti-LAMP1 antibody
| Name | Anti-LAMP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62562 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A 15 amino acid peptide from near the centre of human LAMP1 |
| Description | Rabbit Polyclonal |
| Gene | LAMP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/mlLane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/mlLane 1 : EL4 cell lysateLane 2 : EL4 cell lysateLysates/proteins at 15 µg per lane.
Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunofluorescence of LAMP-1 in Human Colon cells using ab62562 at 20 ug/ml.
ab62562, at 20µg/ml, staining LAMP1 in human colon tissue by immunofluorescence.
Product References
Transgenic expression of intraneuronal Abeta42 but not Abeta40 leads to cellular - Transgenic expression of intraneuronal Abeta42 but not Abeta40 leads to cellular
Abramowski D, Rabe S, Upadhaya AR, Reichwald J, Danner S, Staab D, Capetillo-Zarate E, Yamaguchi H, Saido TC, Wiederhold KH, Thal DR, Staufenbiel M. J Neurosci. 2012 Jan 25;32(4):1273-83.
Discrete domains of MARCH1 mediate its localization, functional interactions, and - Discrete domains of MARCH1 mediate its localization, functional interactions, and
Jabbour M, Campbell EM, Fares H, Lybarger L. J Immunol. 2009 Nov 15;183(10):6500-12.