Anti-LAMP1 antibody [H4A3]
| Name | Anti-LAMP1 antibody [H4A3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab25630 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | H4A3 |
| Applications | ICC/IF ICC/IF FC IHC-F WB IHC-P |
| Species Reactivities | Rat, Human, Monkey |
| Antigen | The details of the immunogen for this antibody are not available |
| Description | Mouse Monoclonal |
| Gene | LAMP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Jurkat cells stained with ab25630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25630, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14972_LAMP1-Primary-antibodies-ab25630-14.jpg)
Overlay histogram showing Jurkat cells stained with ab25630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25630, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab25630 at 1/100 staining human Primary Gingival epithelial cells by ICC/IF. The cells were ixed with 4% paraformaldehyde, permeabilized with 0.5% saponin and then blocked overnight with 10% goat serum, 5% BSA. The cells were incubated with the antibody for 1 hour and then a FITC conjugated goat polyclonal antibody was used as the secondary. The cells were counterstaind with DAPI for the nucleus and Cell Tracker Blue for the cytoplasm.See Abreview
Ab25630 staining Human normal placenta. Staining is localized to the cell membrane.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab25630 staining LAMP1 in human HaCaT keratinocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with acetone, permeabilized with ice cold acetone and blocking with 4% PBS, 0.4% BSA and goat serum was performed for 16 hours at 40C. Samples were incubated with primary antibody (1/100: in 10% blocking solution in PBS) for 1 hour at 230C. An Alexa Fluor ® 680-conjugated goat polyclonal to mouse IgG was used at dilution at 1/200 as secondary antibody. Alexafluor-680 signal is pseudocolored green in the image.See Abreview
ab25630 at 1/500 dilution staining LAMP1 in human gastric cancer cells by immunocytochemistry/ immunofluorescence. Sections were methanol fixed, permeabilized in 0.5% Triton X-100 prior to blocking in 10% NGS/1% BSA for 1 hour at 25°C and then incubated with ab25630 for 2 hours at 25°C. Alexa fluor® 594 mouse polyclonal to mouse Ig, diluted 1/600, was used as the secondary antibody.See Abreview
IHC-Fr image of human brain section stained with ab25630. The brain sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25630, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.See Abreview
![All lanes : Anti-LAMP1 antibody [H4A3] (ab25630) at 1/10000 dilutionLane 1 : Iron treated 3 month old liver at 20 µgLane 2 : Untreated 3 month old liver at 20 µgLane 3 : One month old untreated liverdeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14978_LAMP1-Primary-antibodies-ab25630-13.jpg)
All lanes : Anti-LAMP1 antibody [H4A3] (ab25630) at 1/10000 dilutionLane 1 : Iron treated 3 month old liver at 20 µgLane 2 : Untreated 3 month old liver at 20 µgLane 3 : One month old untreated liverdeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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