Anti-LAMP2b antibody - Lysosome Marker

• Supplier: Abcam
• Catalog: ab18529
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Bovine, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human LAMP2b
• Blocking Peptide: Human LAMP2b peptide
• Description: Rabbit Polyclonal
• Gene: LAMP2
• Conjugate: Unconjugated

Product images

All lanes : Anti-LAMP2b antibody - Lysosome Marker (ab18529) at 1 µg/mlLane 1 : Human skeletal muscle lysateLane 2 : Human liver lysate Lane 3 : Human skeletal muscle lysate with Human LAMP2b peptide (ab23321) at 1 µg/mlLane 4 : Human liver lysate with Human LAMP2b peptide (ab23321) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

ICC/IF image of ab18529 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18529, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

Lanes 1 - 3 : Anti-LAMP2b antibody - Lysosome Marker (ab18529) at 1 µg/mlLanes 4 - 5 : Non Abcam anti-LAMP2 antibodyLane 1 : MEF cells extracted using 0.5% NP40Lane 2 : HeLa cells extracted using 1% Triton X-100Lane 3 : HeLa cells extracted using 0.5% NP40Lane 4 : MEF cells extracted using 1% Triton X-100Lane 5 : MEF cells extracted using 0.5% NP40SecondaryGoat anti-rabbit HRP at 1/20000 dilutionPerformed under reducing conditions.

IHC image of ab18529 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18529, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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