LAMP2

Gene Symbol LAMP2
Entrez Gene 3920
Alt Symbol CD107b, LAMP-2, LAMPB, LGP110
Species Human
Gene Type protein-coding
Description lysosomal-associated membrane protein 2
Other Description CD107 antigen-like family member B|lysosome-associated membrane glycoprotein 2
Swissprots Q99534 Q96J30 Q6Q3G8 A8K4X5 Q16641 P13473 D3DTF0 Q9UD93
Accessions AAB41647 AAB67313 AAB67314 EAX11881 EAX11882 EAX11883 EAX11884 P13473 AK291090 BAF83779 AK293931 BAG57310 AK295038 BAH11955 AK304405 BAG65239 AY561849 AAS67876 BC002965 AAH02965 BC040653 BP386088 DA405328 DQ892095 ABM83021 DQ895288 ABM86214 J04183 AAA60383 S79873 AAB35426 U36336 AAA91149 X77196 CAA54416 NM_001122606 NP_001116078 NM_002294 NP_002285 NM_013995 NP_054701
Function Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. {ECO:0000269|PubMed:8407947}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255|PROSITE- ProRule:PRU00740, ECO:0000269|PubMed:17897319}. Endosome membrane {ECO:0000269|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255|PROSITE-ProRule:PRU00740, ECO:0000269|PubMed:17897319}. Lysosome membrane {ECO:0000255|PROSITE-ProRule:PRU00740, ECO:0000269|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255|PROSITE-ProRule:PRU00740, ECO:0000269|PubMed:17897319}. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Tissue Specificity Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Top Pathways Lysosome, Phagosome, Tuberculosis

LAMP-2 (ABL-93) - sc-20004 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
LAMP-2 (ABL-93): sc-20004. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.

LAMP-2 (C-20) - sc-8100 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LAMP-2 (D-20) - sc-34241 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LAMP-2 (H-207) - sc-5571 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LAMP-2 (H-207): sc-5571. Western blot analysis of LAMP-2 expression in U-937 whole cell lysate.

LAMP-2 (H4B4) - sc-18822 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LAMP-2 siRNA (h): sc-29390. Immunofluorescence staining of methanol-fixed, control HeLa (A) and LAMP-2 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with LAMP-2 (H4B4): sc-18822.

LAMP-2 (N-17) - sc-8101 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LAMP-2 (N-17): sc-8101. Western blot analysis of LAMP-2 expression in ECV304 (A) and T24 (B) whole cell lysates.

Anti-LAMP2 antibody - ab101325 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-LAMP2 antibody (ab101325) at 1/3000 dilution + HCT116 whole cell lysate at 30 µg

Anti-LAMP2 antibody - ab71066 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-LAMP2 antibody (ab71066) at 1/60 dilution + Y79 cell line lysate at 35 µg/ml

Anti-LAMP2 antibody [H4B4] - ab25631 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone H4B4
ab25631 at 0.5µg/ml staining human HEK cells by immunocytochemistry. The cells were fixed with paraformaldehyde and incubated with the antibody for 1 hour. Bound antibody was detected using a goat anti-mouse IgG Alexa-Fluor ® 568. In the confocal image ab25631 labelling in red shows a distribution consistent with the location of lysosomes. Blue nuclear counterstain is present.This image is courtesy of an Abreview submitted by Randal Moldrich on 31 March 2006.See Abreview

Anti-LAMP2 antibody [H4B4] - ab119124 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone H4B4
IHC image of LAMP2 staining in human skin melanoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-LAMP2 antibody [H4B4] - ab112019 from Abcam

Prices $435.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone H4B4
Immunohistochemical analysis of LAMP2 expression in formalin-fixed, paraffin-embedded Human breast tissue using ab112019 at 20 µg/ml.

Anti-LAMP2 antibody [H4B4] (Alexa Fluor® 488) - ab187607 from Abcam

Prices $370.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2 antibody [H4B4] (Allophycocyanin) - ab136302 from Abcam

Prices $370.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2 antibody [H4B4] (Biotin) - ab25447 from Abcam

Prices $370.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2 antibody [H4B4] (FITC) - ab25407 from Abcam

Prices $188.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone H4B4
Overlay histogram showing THP1 cells stained with ab25407 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab254071, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-LAMP2 antibody [H4B4] (FITC) - ab176550 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2 antibody [H4B4] (PE/Cy5®) - ab25223 from Abcam

Prices $185.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2 antibody [H4B4] (Phycoerythrin) - ab25368 from Abcam

Prices $186.00
Sizes 50 tests
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2 antibody [H4B4], prediluted (Phycoerythrin) - ab178546 from Abcam

Prices $370.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone H4B4

Anti-LAMP2a antibody - ab18528 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab18528 stained MEF1 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18528 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-LAMP2a antibody [EPR4207(2)] - Lysosome Marker - ab125068 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4207(2)
All lanes : Anti-LAMP2a antibody [EPR4207(2)] - Lysosome Marker (ab125068) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : ECV304 cell lysateLane 4 : JAR cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-LAMP2a antibody [EPR4207(2)] - Lysosome Marker (Alexa Fluor® 488) - ab184808 from Abcam

Prices $465.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4207(2)
Overlay histogram showing HeLa cells stained with ab184808 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184808, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-LAMP2b antibody - ab118959 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118959 staining LAMP2b in Human DU145 prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF568-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-LAMP2b antibody - Lysosome Marker - ab18529 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LAMP2b antibody - Lysosome Marker (ab18529) at 1 µg/mlLane 1 : Human skeletal muscle lysateLane 2 : Human liver lysate Lane 3 : Human skeletal muscle lysate with Human LAMP2b peptide (ab23321) at 1 µg/mlLane 4 : Human liver lysate with Human LAMP2b peptide (ab23321) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

Anti-LAMP2 / CD107b Antibody (aa29-192) - LS-C295230 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant LAMP2 / CD107b.
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