Western blot analysis of c-Myc expression in HeLa (A,D,F), Jurkat (B), K-562 (C) and NIH/3T3 (G) whole cell lysates and Jurkat (E,H) nuclear extracts. Antibodies tested include c-Myc (9E10): sc-40 (A-C), c-Myc (C-33): sc-42 (D,E) and c-Myc (N-262): sc-764 (F-H).
c-Myc (9E10): sc-40. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining.
c-Myc (9E10): sc-40. Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells.
c-Myc (9E10) FITC: sc-40 FITC. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2855.
c-Myc (9E10): sc-40. Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.
pCruz Myc: sc-5043. Western blot analysis of c-Myc-Lac Z fusion protein expression in COS cells transfected with pCruz Myc-Lac Z (A), pCruz His-Lac Z (B) and non-transfected COS cells (C). Blot probed with c-Myc (9E10): sc-40.
c-Myc siRNA (h): sc-29226. Western blot analysis of c-Myc expression in non-transfected control (A) and c-Myc siRNA transfected (B) HeLa cells. Blot probed with c-Myc (9E10): sc-40. Actin (I-19): sc-1616 used as specificity and loading control.
c-Myc (9E10) Alexa Fluor 488: sc-40 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-3890.
c-Myc (9E10) Alexa Fluor 647: sc-40 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-24636.
c-Myc (9E10): sc-40. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-110502 (B) 293T whole cell lysates.
c-Myc (9E10): sc-40. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-116671 (B) 293T whole cell lysates.
c-Myc (9E10): sc-40. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-110502 (B) 293T whole cell lysates.
c-Myc (9E10): sc-40. Immunofluorescence staining of methanol-fixed untransfected (A) and c-myc tag transfected HEK 293T cells (B).
Western blot analysis of c-Myc expression in untreated (A) and (E)-4-Hydroxyhexenal (sc-202593) treated (B) Jurkat whole cell lysates. Antibodies tested include c-Myc (9E10): sc-40 (A,B). Note down regulation of c-Myc in lane B.
c-Myc Nickase Plasmid (h): sc-400001-NIC. Western blot analysis of c-Myc expression in non-transfected control (A) and c-Myc Nickase Plasmid transfected (B) HEK293T whole cell lysates. Blot probed with c-Myc (9E10): sc-40. β-Actin (C4): sc-47778 used as specificity and loading control.