Anti-LAP1 antibody [RL13]

• Supplier: Abcam
• Catalog: ab2737
• Prices: $370.00
• Sizes: 200 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: RL13
• Applications: IHC-P ICC/IF ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Other Immunogen Type corresponding to Rat LAP1
• Description: Mouse Monoclonal
• Gene: LRRC7
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on rat breast tissue. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 (1:50) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on rat colon tissue. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 (1:50) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on rat lymph node tissue. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 (1:50) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

Immunocytochemistry/Immunofluorescence analysis of LAP1 using ab2737 shows staining in NS-1 Cells.

Product References

Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine - Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine

Liao JJ, Huang MC, Graler M, Huang Y, Qiu H, Goetzl EJ. J Biol Chem. 2007 Jan 19;282(3):1964-72. Epub 2006 Nov 22.

Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the - Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the

Yang L, Guan T, Gerace L. J Cell Biol. 1997 Dec 1;139(5):1077-87.

Structural analysis of the p62 complex, an assembly of O-linked glycoproteins - Structural analysis of the p62 complex, an assembly of O-linked glycoproteins

Guan T, Muller S, Klier G, Pante N, Blevitt JM, Haner M, Paschal B, Aebi U, Gerace L. Mol Biol Cell. 1995 Nov;6(11):1591-603.

cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an - cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an

Martin L, Crimaudo C, Gerace L. J Biol Chem. 1995 Apr 14;270(15):8822-8.

Integral membrane proteins of the nuclear envelope interact with lamins and - Integral membrane proteins of the nuclear envelope interact with lamins and

Foisner R, Gerace L. Cell. 1993 Jul 2;73(7):1267-79.

Integral membrane proteins specific to the inner nuclear membrane and associated - Integral membrane proteins specific to the inner nuclear membrane and associated

Senior A, Gerace L. J Cell Biol. 1988 Dec;107(6 Pt 1):2029-36.