Western blot analysis of human recombinant c-Myc fusion protein untreated (A,C) or phosphorylated by p38α (B,D). Antibodies tested include c-Myc (N-262): sc-764 (A,B) and p-c-Myc (Thr 58/Ser 62)-R: sc-8000-R (C,D).
Western blot analysis of c-Myc expression in HeLa (A,D,F), Jurkat (B), K-562 (C) and NIH/3T3 (G) whole cell lysates and Jurkat (E,H) nuclear extracts. Antibodies tested include c-Myc (9E10): sc-40 (A-C), c-Myc (C-33): sc-42 (D,E) and c-Myc (N-262): sc-764 (F-H).
c-Myc (N-262): sc-764. Western blot analysis of c-Myc expression in COS (A) and Jurkat (B) whole cell lysates.
c-Myc (N-262): sc-764. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and mouse c-Myc transfected: sc-118892 (B) 293T whole cell lysates.
c-Myc (N-262): sc-764. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and human c-Myc transfected: sc-116671 (B) 293T whole cell lysates.
c-Myc (N-262): sc-764. Nuclear c-Myc in small intestinal adenoma in APC -/+ (20X microscopic magnification). Dilution: 1:80 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. Kindly provided by Dr. Albert J. Fornace Jr., Georgetown University.
Western blot analysis of c-Myc phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Myc transfected: sc-118892 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse c-Myc transfected: sc-118892 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Myc (C-3): sc-377551 (A,B,C) and c-Myc (N-262): sc-764 (D,E,F).
Western blot analysis of c-Myc phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Myc transfected: sc-118892 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse c-Myc transfected: sc-118892 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Myc (E-3): sc-377552 (A,B,C) and c-Myc (N-262): sc-764 (D,E,F).
Western blot analysis of c-Myc phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Myc transfected: sc-118892 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse c-Myc transfected: sc-118892 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Myc (Thr 58/Ser 62)-R: sc-8000-R (A,B,C) and c-Myc (N-262): sc-764 (D,E,F).