Anti-LATS2 antibody
| Name | Anti-LATS2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70565 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IP |
| Species Reactivities | Mouse, Human, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Chimpanzee, Ape, Orangutan, Xenopus, Platypus |
| Antigen | Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human LATS2 (NP_055387 |
| Description | Rabbit Polyclonal |
| Gene | LATS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-LATS2 antibody (ab70565) at 0.4 µg/mlLane 1 : HeLa cell whole lysate at 50 µgLane 2 : HeLa cell whole lysate at 15 µgLane 3 : HeLa cell whole lysate at 5 µg
Detection of human LATS2 by Immunoprecipitation in HeLa whole cell lysate (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70565 at 3 µg/mg of lysate. Subsequent blotting of immunoprecipitate for the detection of LATS2 was performed using 1µg/ml of ab70565. Detection was by chemiluminescence.
ab70565 (2µg/ml) staining LATS2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in pneumocytes.Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
LATS2 is a modulator of estrogen receptor alpha. - LATS2 is a modulator of estrogen receptor alpha.
Lit LC, Scott S, Zhang H, Stebbing J, Photiou A, Giamas G. Anticancer Res. 2013 Jan;33(1):53-63.
LATS2 is a tumor suppressor gene of malignant mesothelioma. - LATS2 is a tumor suppressor gene of malignant mesothelioma.
Murakami H, Mizuno T, Taniguchi T, Fujii M, Ishiguro F, Fukui T, Akatsuka S, Horio Y, Hida T, Kondo Y, Toyokuni S, Osada H, Sekido Y. Cancer Res. 2011 Feb 1;71(3):873-83.