LATS2

Gene Symbol LATS2
Entrez Gene 26524
Alt Symbol KPM
Species Human
Gene Type protein-coding
Description large tumor suppressor kinase 2
Other Description LATS (large tumor suppressor, Drosophila) homolog 2|LATS, large tumor suppressor, homolog 2|kinase phosphorylated during mitosis protein|large tumor suppressor homolog 2|serine/threonine kinase KPM|serine/threonine-protein kinase LATS2|serine/threonine-protein kinase kpm|warts-like kinase
Swissprots Q9P2X1 B1AM47 Q9NRM7
Accessions EAX08286 EAX08287 Q9NRM7 AB028019 BAA92381 AB209897 BAD93134 AF207547 AAF80561 AK123520 AK314235 BAG36905 BC036437 BC071572 BC127671 BC156423 AAI56424 BC172545 AAI72545 BQ774580 DB452765 XM_005266342 XP_005266399 XM_011535042 XP_011533344 NM_014572 NP_055387
Function Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilizatio
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Note=Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.
Tissue Specificity Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. {ECO:0000269|PubMed:10673337, ECO:0000269|PubMed:10871863}.
Top Pathways Hippo signaling pathway

LATS2 (D83D6) Rabbit mAb - 5888 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D83D6
Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with human LATS2 (+), using LATS2 (D83D6) Rabbit mAb.

LATS2 Antibody - 13646 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using LATS2 Antibody.

kpm (K-17) - sc-23065 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
kpm (k-17): sc-23065. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

kpm (H-14) - sc-23067 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
kpm (H-14): sc-23067. Western blot analysis of kpm expression in mouse lung (A) and rat brain (B) tissue extracts and SK-OV-3 (C) and A549 (D) whole cell lysates.

Anti-LATS1 +LATS2 (phospho T1079 + T1041) antibody - ab111344 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111344 at 1/50 dilution staining LATS1 +LATS2 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-LATS2 antibody - ab70565 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LATS2 antibody (ab70565) at 0.4 µg/mlLane 1 : HeLa cell whole lysate at 50 µgLane 2 : HeLa cell whole lysate at 15 µgLane 3 : HeLa cell whole lysate at 5 µg

Anti-LATS2 antibody - ab111054 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111054 at 1/100 dilution staining LATS2 in HepG2 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

Anti-LATS2 antibody - ab135794 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-LATS2 antibody (ab135794) at 1/100 dilution + NIH/3T3 cell lysate at 12.5 µg

Anti-LATS2 antibody - ab110780 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LATS2 antibody (ab110780) at 1 µg/mlLane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-LATS2 antibody [mAbcam54073] - ab54073 from Abcam

Host Mouse
Clonality Monoclonal
Clone mAbcam54073
Anti-LATS2 antibody [mAbcam54073] (ab54073) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-LATS2 antibody [mAbcam54073] (Alexa Fluor® 488) - ab197279 from Abcam

Host Mouse
Clonality Monoclonal
Clone mAbcam54073
ab197279 staining LATS2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197279 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-LATS2 antibody [mAbcam54073] (Alexa Fluor® 647) - ab197280 from Abcam

Host Mouse
Clonality Monoclonal
Clone mAbcam54073
ab197280 staining LATS2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197280 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-LATS2 Antibody - LS-C71552 from LifeSpan Bioscience

Prices $635.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (aa1000-1027, AP) - LS-C261735 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (aa1000-1027, APC) - LS-C261737 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (aa1000-1027, FITC) - LS-C261738 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (aa1000-1027, Azide-free, HRP) - LS-C261739 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (aa1000-1027, Biotin) - LS-C261750 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (Internal, AP) - LS-C278868 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (Internal, APC) - LS-C278873 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (Internal, Biotin) - LS-C278874 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (Internal, Azide-free, HRP) - LS-C278875 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (aa541-590) - LS-C199192 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human skeletal muscle tissue, using LATS2 Antibody. The sample on the right was incubated with synthetic peptide.

Anti-LATS2 Antibody (Internal, FITC) - LS-C278876 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LATS2 Antibody (Internal) - LS-C330504 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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