Anti-LATS2 antibody [mAbcam54073]
| Name | Anti-LATS2 antibody [mAbcam54073] |
|---|---|
| Supplier | Abcam |
| Catalog | ab54073 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | mAbcam54073 |
| Applications | ICC/IF ICC/IF WB IHC-P FC |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LATS2 |
| Blocking Peptide | Human LATS2 peptide |
| Description | Mouse Monoclonal |
| Gene | LATS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-LATS2 antibody [mAbcam54073] (ab54073) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/15279_ab54073_1.jpg)
Anti-LATS2 antibody [mAbcam54073] (ab54073) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC image of ab54073 staining LATS2 in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54073, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab54073 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54073, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab54073 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54073, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/15282_LATS2-Primary-antibodies-ab54073-3.jpg)
Overlay histogram showing HeLa cells stained with ab54073 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54073, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
A functional interaction between Hippo-YAP signalling and FoxO1 mediates the - A functional interaction between Hippo-YAP signalling and FoxO1 mediates the
Shao D, Zhai P, Del Re DP, Sciarretta S, Yabuta N, Nojima H, Lim DS, Pan D, Sadoshima J. Nat Commun. 2014;5:3315.
MicroRNA-31 regulated by the extracellular regulated kinase is involved in - MicroRNA-31 regulated by the extracellular regulated kinase is involved in
Liu X, Cheng Y, Chen X, Yang J, Xu L, Zhang C. J Biol Chem. 2011 Dec 9;286(49):42371-80.