Anti-LC3A/B antibody (ab128025) at 1/1000 dilution + Mouse heart tissue cell lysate at 30 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-LC3A/B antibody (ab128025) at 2 µg/mlLane 1 : WTLane 2 : atg5-/-
ab128025 staining LC3A/B in MEF cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.See Abreview