Anti-LDL Receptor antibody [EP1553Y]
| Name | Anti-LDL Receptor antibody [EP1553Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52818 |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1553Y |
| Applications | ICC/IF ICC/IF WB FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to residues from the C-terminus of the human LDL receptor |
| Description | Rabbit Monoclonal |
| Gene | LDLR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab52818 at 1/100 dilution staining LDL receptor in HepG2 cells by Immunocytochemistry.
![Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human Plasma Total Protein Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/15537_LDL-Receptor-Primary-antibodies-ab52818-2.jpg)
Anti-LDL Receptor antibody [EP1553Y] (ab52818) at 1/500 dilution + Human Plasma Total Protein Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Overlay histogram showing U937 cells stained with ab52818 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52818, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ICC/IF image of ab52818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52818, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Reduction of VLDL secretion decreases cholesterol excretion in niemann-pick - Reduction of VLDL secretion decreases cholesterol excretion in niemann-pick
Marshall SM, Kelley KL, Davis MA, Wilson MD, McDaniel AL, Lee RG, Crooke RM, Graham MJ, Rudel LL, Brown JM, Temel RE. PLoS One. 2014 Jan 3;9(1):e84418.
KIF13B enhances the endocytosis of LRP1 by recruiting LRP1 to caveolae. - KIF13B enhances the endocytosis of LRP1 by recruiting LRP1 to caveolae.
Kanai Y, Wang D, Hirokawa N. J Cell Biol. 2014 Feb 3;204(3):395-408.
Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin - Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin
Niesman IR, Schilling JM, Shapiro LA, Kellerhals SE, Bonds JA, Kleschevnikov AM, Cui W, Voong A, Krajewski S, Ali SS, Roth DM, Patel HH, Patel PM, Head BP. J Neuroinflammation. 2014 Mar 3;11:39.
A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is - A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is
Hu YW, Yang JY, Ma X, Chen ZP, Hu YR, Zhao JY, Li SF, Qiu YR, Lu JB, Wang YC, Gao JJ, Sha YH, Zheng L, Wang Q. J Lipid Res. 2014 Apr;55(4):681-97.
Hepatic ACAT2 knock down increases ABCA1 and modifies HDL metabolism in mice. - Hepatic ACAT2 knock down increases ABCA1 and modifies HDL metabolism in mice.
Pedrelli M, Davoodpour P, Degirolamo C, Gomaraschi M, Graham M, Ossoli A, Larsson L, Calabresi L, Gustafsson JA, Steffensen KR, Eriksson M, Parini P. PLoS One. 2014 Apr 2;9(4):e93552.
Targeted disruption of LDLR causes hypercholesterolemia and atherosclerosis in - Targeted disruption of LDLR causes hypercholesterolemia and atherosclerosis in
Davis BT, Wang XJ, Rohret JA, Struzynski JT, Merricks EP, Bellinger DA, Rohret FA, Nichols TC, Rogers CS. PLoS One. 2014 Apr 1;9(4):e93457.
Strong improvement of apolipoprotein E/low-density lipoprotein receptor signals - Strong improvement of apolipoprotein E/low-density lipoprotein receptor signals
Yamashita T, Zhai Y, Kurata T, Hishikawa N, Morimoto N, Ohta Y, Deguchi K, Abe K. J Stroke Cerebrovasc Dis. 2014 Oct;23(9):2240-9. doi:
High fat diet modulates Trypanosoma cruzi infection associated myocarditis. - High fat diet modulates Trypanosoma cruzi infection associated myocarditis.
Nagajyothi F, Weiss LM, Zhao D, Koba W, Jelicks LA, Cui MH, Factor SM, Scherer PE, Tanowitz HB. PLoS Negl Trop Dis. 2014 Oct 2;8(10):e3118.
Aggregated low-density lipoprotein induces LRP1 stabilization through E3 - Aggregated low-density lipoprotein induces LRP1 stabilization through E3
Cal R, Garcia-Arguinzonis M, Revuelta-Lopez E, Castellano J, Padro T, Badimon L, Llorente-Cortes V. Arterioscler Thromb Vasc Biol. 2013 Feb;33(2):369-77. doi:
PCSK9-mediated degradation of the LDL receptor generates a 17 kDa C-terminal LDL - PCSK9-mediated degradation of the LDL receptor generates a 17 kDa C-terminal LDL
Tveten K, Strom TB, Berge KE, Leren TP. J Lipid Res. 2013 Jun;54(6):1560-6.