Anti-LEF1 antibody [EPR2029Y]

Name	Anti-LEF1 antibody [EPR2029Y]
Supplier	Abcam
Catalog	ab137872
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR2029Y
Applications	WB IHC-P ICC/IF ICC/IF IHC-F
Species Reactivities	Rat, Human
Antigen	Synthetic peptide corresponding to residues in Human LEF1
Description	Rabbit Monoclonal
Gene	LEF1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/2000 dilution (purified) + Rat thymus tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/10000 dilution (purified) + Jurkat cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/1000 dilution (purified) + Human fetal thymus lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded rat spleen with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded human thymus with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded human tonsil with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescence staining of Jurkat cells with purified ab137872 at a working dilution of 1 in 500, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab137872 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LEF1 with unpurified ab137872 at 1/100.

Product References

Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for - Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for

Skokowa J, Klimiankou M, Klimenkova O, Lan D, Gupta K, Hussein K, Carrizosa E, Kusnetsova I, Li Z, Sustmann C, Ganser A, Zeidler C, Kreipe HH, Burkhardt J, Grosschedl R, Welte K. Nat Med. 2012 Oct;18(10):1550-9.

Nuclear overexpression of lymphoid-enhancer-binding factor 1 identifies chronic - Nuclear overexpression of lymphoid-enhancer-binding factor 1 identifies chronic

Tandon B, Peterson L, Gao J, Nelson B, Ma S, Rosen S, Chen YH. Mod Pathol. 2011 Nov;24(11):1433-43.