LEF1

Gene Symbol LEF1
Entrez Gene 51176
Alt Symbol LEF-1, TCF10, TCF1ALPHA, TCF7L3
Species Human
Gene Type protein-coding
Description lymphoid enhancer-binding factor 1
Other Description T cell-specific transcription factor 1-alpha|TCF1-alpha
Swissprots B4DG38 Q9UJU2 Q3ZCU4 B7Z8E2 E9PDK3 Q9HAZ0
Accessions AAK64602 AAN06089 AEW16365 EAX06221 EAX06222 EAX06223 EAX06224 EAX06225 Q9UJU2 AF086339 AF198532 AAF13268 AF288571 AAG01022 AF294627 AAG26886 AK128255 AK225772 AK294395 BAG57649 AK303143 BAG64247 AK303272 BAH13928 AL049409 CAH56421 BC040559 AAH40559 BC050632 AAH50632 DC405840 DC406065 EU446873 ABZ92402 XM_005263046 XP_005263103 XM_005263047 XP_005263104 XM_005263048 XP_005263105 XM_006714233 XP_006714296 NM_001130713 NP_001124185 NM_001130714 NP_001124186 NM_001166119 NP_001159591 NM_016269 NP_057353
Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. {ECO:0000250, ECO:0000269|PubMed:11266540, ECO:0000269|PubMed:19653
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding. {ECO:0000250}.
Tissue Specificity Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. {ECO:0000269|PubMed:19653274}.
Top Pathways Hippo signaling pathway, Wnt signaling pathway, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Thyroid cancer, Prostate cancer

LEF1 (C12A5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 14022 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C12A5
Flow cytometric analysis of Jurkat cells using LEF1 (C12A5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 647 Conjugate) #2985 (red).

LEF1 (C12A5) Rabbit mAb (PE Conjugate) - 14440 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C12A5
Flow cytometric analysis of Jurkat cells using LEF1 (C12A5) Rabbit mAb (PE Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (PE Conjugate) #5742 (red).

LEF1 (C12A5) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 8490 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C12A5
Flow cytometric analysis of Jurkat cells using LEF1 (C12A5) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate) #2975 (red).

LEF1 (C18A7) Rabbit mAb - 2286 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C18A7
Western blot analysis of total cell lysates from mouse thymocytes, DLD1 and HCT15 cells, using LEF1 (C18A7) Rabbit mAb.

LEF1 (C12A5) Rabbit mAb - 2230 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C12A5
Western blot analysis of total cell lysates from HCT15, DLD1 and mouse thymocytes using LEF1 (C12A5) Rabbit mAb.

LEF-1 (B-10) - sc-374412 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LEF-1 (B-10): sc-374412. Western blot analysis of LEF-1 expression in non-transfected: sc-117752 (A) and human LEF-1 transfected: sc-116288 (B) 293T whole cell lysates.

LEF-1 (B-6) - sc-374522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LEF-1 (B-6): sc-374522. Western blot analysis of LEF-1 expression in non-transfected: sc-117752 (A) and human LEF-1 transfected: sc-116288 (B) 293T whole cell lysates.

LEF-1 (C-19) - sc-8592 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ChIP analysis of transcription regulatory proteins associated with the Pitx2 promoter in aT3-1 cells in response to lithium. Antibodies tested include LEF-1 (C-19): sc-8592 amd LEF-1 (N-17): sc-8591 (B), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (C), HDAC1 (H-11): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (D), HDAC2 (C-8): sc-9959, HDAC2 (H-54): sc-7899 and HDAC2 (C-19): sc-6296 (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.

LEF-1 (H-70) - sc-28687 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LEF-1 (H-70): sc-28687. Western blot analysis of LEF-1 expression in non-transfected: sc-117752 (A) and human LEF-1 transfected: sc-116288 (B) 293T whole cell lysates.

LEF-1 (N-17) - sc-8591 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ChIP analysis of transcription regulatory proteins associated with the Pitx2 promoter in aT3-1 cells in response to lithium. Antibodies tested include LEF-1 (C-19): sc-8592 amd LEF-1 (N-17): sc-8591 (B), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (C), HDAC1 (H-11): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (D), HDAC2 (C-8): sc-9959, HDAC2 (H-54): sc-7899 and HDAC2 (C-19): sc-6296 (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.

LEF-1 (REMB1) - sc-56722 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
LEF-1 (REMB1): sc-56722. Western blot analysis of LEF-1 expression in Jurkat whole cell lysate.

LEF-1 (2D12) - sc-81470 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
LEF-1 (2D12): sc-81470. Western blot analysis of LEF-1 expression in non-transfected 293T: sc-117752 (A), human LEF-1 transfected 293T: sc-116288 (B) and Jurkat (C) whole cell lysates.

Anti-LEF1 (phospho S42) antibody - ab74067 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LEF1 (phospho S42) antibody (ab74067) at 1/500 dilutionLane 1 : 293 cell extracts treated with paclitaxel (1uM, 24hours)Lane 2 : 293 cell extracts treated with paclitaxel (1uM, 24hours) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-LEF1 antibody - ab52017 from Abcam

Prices $378.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-LEF1 antibody (ab52017) at 1 µg/ml + Daudi cell lysate (35µg protein in RIPA buffer).

Anti-LEF1 antibody - ab22884 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-LEF1 antibody (ab22884) at 2 µg/ml + Jurkat cell lysate with 5% skim milk in PBSSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-LEF1 antibody - ab85052 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab85052 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85052 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-LEF1 antibody - ab74549 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-LEF1 antibody (ab74549) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-LEF1 antibody - ab124271 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LEF1 antibody (ab124271) at 0.4 µg/mlLane 1 : Jurkat whole cell lysate at 50 µgLane 2 : Jurkat whole cell lysate at 15 µgdeveloped using the ECL techniqueExposure time : 3 minutes

Anti-LEF1 antibody - ab83964 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-LEF1 antibody (ab83964) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-LEF1 antibody - ab28316 from Abcam

Prices $376.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
All lanes : Anti-LEF1 antibody (ab28316) at 5 µg/mlLane 1 : MockLane 2 : TCF1Lane 3 : TCF4Lane 4 : Jurkat cell extractLane 5 : Full length LEF1Lane 6 : Full length LEF1 Ø HMGLane 7 : Recombinant full length LEF1 at 0.01 µgLane 8 : LEF1 amino acids 1 - 192Lane 9 : LEF1 amino acids 1 - 276

Anti-LEF1 antibody [1C3] - ab12038 from Abcam

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1C3

Anti-LEF1 antibody [EP2030Y] - ab53293 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2030Y
Anti-LEF1 antibody [EP2030Y] (ab53293) at 1/500000 dilution + Jurkat cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Anti-LEF1 antibody [EPR2029Y] - ab137872 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2029Y
Anti-LEF1 antibody [EPR2029Y] (ab137872) at 1/2000 dilution (purified) + Rat thymus tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-LEF1 antibody [REMB1] - ab12034 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone REMB1

Anti-LEF1 antibody [REMB6] - ab12035 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone REMB6
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