Anti-Lefty antibody
| Name | Anti-Lefty antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22569 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty |
| Description | Rabbit Polyclonal |
| Gene | LEFTY1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab22569 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22569, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-Lefty antibody (ab22569) at 1 µg/mlLane 1 : Mouse Lefty1 Recombinant ProteinLane 2 : Human LeftyA Recombinant ProteinLysates/proteins at 0.01 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilution