Anti-LEO1 antibody
| Name | Anti-LEO1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33157 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Rat, Chicken, Dog, Xenopus |
| Antigen | Synthetic peptide derived from within residues 300 - 400 of Human LEO1 |
| Description | Rabbit Polyclonal |
| Gene | LEO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-LEO1 antibody (ab33157) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab33157 staining LEO1 in Mouse ES cells by Immunocytochemistry/ Immunofluorescence. The cells were methanol fixed and blocked in 0.2% Fish scale gelatin for 1 hour at 25°C. Primary antibody was used at 1/100 dilution (0.2% Fish scale gelatin in PBS) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® 488 conjugated Donkey polyclonal to rabbit IgG was used as secondary at 1/500 dilution. Image shows green color staining with ab33157 and blue with DAPI. See Abreview
All lanes : Anti-LEO1 antibody (ab33157) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutiondeveloped using the ECL technique