Anti-Lgi1 antibody
| Name | Anti-Lgi1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30868 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Hamster, Human, Chicken, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Lgi1 |
| Description | Rabbit Polyclonal |
| Gene | LGI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Lgi1 antibody (ab30868) at 1/1000 dilution + Rat brain tissue lysate at 30 µgSecondaryHRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Lane 1 : MarkerLanes 2 - 4 : Anti-Lgi1 antibody (ab30868) at 1 µg/mlLane 1 : As aboveLane 2 : Mouse Brain Whole Tissue Lysate at 20 µgLane 3 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µgLane 4 : Rat Brain Whole Tissue Lysate at 20 µgSecondaryLanes 2 - 4 : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ab30868 at 10ug/ml staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked before incubation with the antibody for 16 hours. An Alexa Fluor ® 488 conjugated goat polyclonal antibody was used as the secondary.See Abreview
IHC-FoFr image of Lgi1 (ab30868) staining in rat spinal cord sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique. Image B is a higher power magnification of the Image A, at the level of the interface between the white and grey matter in the lamina V. Numbers in image A are Rexed's laminae numbers.See Abreview
Product References
Glutamatergic neuron-targeted loss of LGI1 epilepsy gene results in seizures. - Glutamatergic neuron-targeted loss of LGI1 epilepsy gene results in seizures.
Boillot M, Huneau C, Marsan E, Lehongre K, Navarro V, Ishida S, Dufresnois B, Ozkaynak E, Garrigue J, Miles R, Martin B, Leguern E, Anderson MP, Baulac S. Brain. 2014 Nov;137(Pt 11):2984-96.
Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize - Autoantibodies to epilepsy-related LGI1 in limbic encephalitis neutralize
Ohkawa T, Fukata Y, Yamasaki M, Miyazaki T, Yokoi N, Takashima H, Watanabe M, Watanabe O, Fukata M. J Neurosci. 2013 Nov 13;33(46):18161-74.
Epilepsy gene LGI1 regulates postnatal developmental remodeling of - Epilepsy gene LGI1 regulates postnatal developmental remodeling of
Zhou YD, Zhang D, Ozkaynak E, Wang X, Kasper EM, Leguern E, Baulac S, Anderson MP. J Neurosci. 2012 Jan 18;32(3):903-10.
Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission - Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission
Fukata Y, Lovero KL, Iwanaga T, Watanabe A, Yokoi N, Tabuchi K, Shigemoto R, Nicoll RA, Fukata M. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3799-804. doi:
ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate - ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate
Ogawa Y, Oses-Prieto J, Kim MY, Horresh I, Peles E, Burlingame AL, Trimmer JS, Meijer D, Rasband MN. J Neurosci. 2010 Jan 20;30(3):1038-48.
Electroclinical characterization of epileptic seizures in leucine-rich, - Electroclinical characterization of epileptic seizures in leucine-rich,
Chabrol E, Navarro V, Provenzano G, Cohen I, Dinocourt C, Rivaud-Pechoux S, Fricker D, Baulac M, Miles R, Leguern E, Baulac S. Brain. 2010 Sep;133(9):2749-62.
A novel three base-pair LGI1 deletion leading to loss of function in a family - A novel three base-pair LGI1 deletion leading to loss of function in a family
de Bellescize J, Boutry N, Chabrol E, Andre-Obadia N, Arzimanoglou A, Leguern E, Baulac S, Calender A, Ryvlin P, Lesca G. Epilepsy Res. 2009 Jul;85(1):118-22.
A novel loss-of-function LGI1 mutation linked to autosomal dominant lateral - A novel loss-of-function LGI1 mutation linked to autosomal dominant lateral
Striano P, de Falco A, Diani E, Bovo G, Furlan S, Vitiello L, Pinardi F, Striano S, Michelucci R, de Falco FA, Nobile C. Arch Neurol. 2008 Jul;65(7):939-42.