Anti-Lipin 1 antibody [EPR3725]

• Supplier: Abcam
• Catalog: ab92316
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3725
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues in human Lipin 1
• Description: Rabbit Monoclonal
• Gene: LPIN1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Lipin 1 antibody [EPR3725] (ab92316) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HepG2 cell lysateLane 3 : HuT-78 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human colon tissue.

ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human uterus tissue.

Immunofluorescent staining of HepG2 cells using ab92316 at 1/100 dilution.

Overlay histogram showing HepG2 cells stained with ab92316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92316, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.