Western blot using affinity purified anti-betaTrCP2 antibody shows detection of mouse and human betaTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1:20,000 in blocking buffer for 1 h at 4 °C.