Anti-Livin antibody [51.3_2A3]

• Supplier: Abcam
• Catalog: ab127979
• Prices: $370.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 51.3_2A3
• Applications: WB IP ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Myeloid Leukemia cells (HL60)
• Description: Mouse Monoclonal
• Gene: BIRC7
• Conjugate: Unconjugated

Product images

ab127979 at 1/100 staining Livin in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.

Lanes 1 - 3 : Anti-Livin antibody [51.3_2A3] (ab127979) at 1/1000 dilutionLanes 4 - 6 : V5 antibody control at 1/1000 dilutionLane 1 : Transfected HeLa cell lysateLane 2 : Non-Transfected HeLa cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : Non-Transfected HeLa cell lysateLane 5 : Transfected HeLa cell lysateLane 6 : SH-SY5Y cell lysate

2 µg of ab127979 was added to aliquots of cleared cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded. Lane 1: Cell Lysate control Lane 2: Cell lysate with ab127979 Lane 3: Cell lysate without ab127979 Lane 4: V5 tag control