ICC/IF image of ab65864 stained JEG-3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-LOX 1 antibody (ab65864) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgLane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188) at 5 µgLane 3 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.