ab140833 staining LOXL2 in mouse tail tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was via the microwave method for 5 minutes. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
ab140833 staining LOXL2 in Human cartilage tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and permeabilized with 0.2% Trioton X-100 in PBS; antigen retrieval was by heat mediation in a sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, pH 6.0). Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Immunohistochemical analysis of formalin-fixed paraffin embedded Human prostate, labelling LOXL2 using ab140833 at 15 μg/ml
Immunohistochemical analysis of formalin-fixed paraffin embedded Human liver, labelling LOXL2 using ab140833 at 15 μg/ml