All lanes : Anti-LOXL2 antibody (ab113201) at 1 µg/mlLane 1 : LNCaP human prostate carcinoma cell line lysate Whole Cell LysateLane 2 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.