Anti-LPP antibody [8B3A11]
| Name | Anti-LPP antibody [8B3A11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab63621 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 8B3A11 |
| Applications | FC WB IHC-P ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Hamster, Human, Monkey |
| Antigen | Purified His-tagged recombinant fragment |
| Description | Mouse Monoclonal |
| Gene | LPP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab63621 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63621, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17120_ab63621-6-ab63621FC.jpg)
Overlay histogram showing HeLa cells stained with ab63621 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63621, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-LPP antibody [8B3A11] (ab63621) at 1/500 dilutionLane 1 : Immunising protein (recombinant fragment)Lane 2 : HeLa cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17121_lpp.jpg)
All lanes : Anti-LPP antibody [8B3A11] (ab63621) at 1/500 dilutionLane 1 : Immunising protein (recombinant fragment)Lane 2 : HeLa cell lysate
![All lanes : Anti-LPP antibody [8B3A11] (ab63621) at 1/2000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from NIH/3T3 cellsLane 3 : Cell lysates prepared from COS cellsLane 4 : Cell lysates prepared from Caki cellsLane 5 : Cell lysates prepared from MCF-7 cellsLane 6 : Cell lysates prepared from HepG2 cellsLane 7 : Cell lysates prepared from SMMC-7721 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/17122_LPP-Primary-antibodies-ab63621-1.jpg)
All lanes : Anti-LPP antibody [8B3A11] (ab63621) at 1/2000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from NIH/3T3 cellsLane 3 : Cell lysates prepared from COS cellsLane 4 : Cell lysates prepared from Caki cellsLane 5 : Cell lysates prepared from MCF-7 cellsLane 6 : Cell lysates prepared from HepG2 cellsLane 7 : Cell lysates prepared from SMMC-7721 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG1
ab63621 at 1/2000 dilution staining LPP in human small intestine tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The DAB staining procedure was used for staining.
ab63621 at 1/2000 dilution staining LPP in human small intestine tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). DAB staining procedure was used for staining.
ab63621 at 1/1000 dilution staining LPP in COS cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. Actin filaments have been stained red with DY-554 phalloidin and blue staining indicates nucleus with DRAQ5 fluorescent DNA dye.
Product References
Modulation of stretch-induced myocyte remodeling and gene expression by nitric - Modulation of stretch-induced myocyte remodeling and gene expression by nitric
Hooper CL, Paudyal A, Dash PR, Boateng SY. Am J Physiol Heart Circ Physiol. 2013 May 15;304(10):H1302-13. doi:
Strong smooth muscle differentiation is dependent on myocardin gene amplification - Strong smooth muscle differentiation is dependent on myocardin gene amplification
Perot G, Derre J, Coindre JM, Tirode F, Lucchesi C, Mariani O, Gibault L, Guillou L, Terrier P, Aurias A. Cancer Res. 2009 Mar 15;69(6):2269-78.