Anti-LRDD antibody

• Supplier: Abcam
• Catalog: ab78389
• Prices: $370.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human, Monkey
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human LRDD
• Description: Rabbit Polyclonal
• Gene: PIDD1
• Conjugate: Unconjugated

Product images

Anti-LRDD antibody (ab78389) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab78389 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78389, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, and MCF-7 cells at 1µg/ml.

Product References

Alternative splicing in osteoclasts and Paget's disease of bone. - Alternative splicing in osteoclasts and Paget's disease of bone.

Klinck R, Laberge G, Bisson M, McManus S, Michou L, Brown JP, Roux S. BMC Med Genet. 2014 Aug 14;15:98.

The calcium-binding protein S100B down-regulates p53 and apoptosis in malignant - The calcium-binding protein S100B down-regulates p53 and apoptosis in malignant

Lin J, Yang Q, Wilder PT, Carrier F, Weber DJ. J Biol Chem. 2010 Aug 27;285(35):27487-98.