Anti-LRP1 antibody [8G1]

• Supplier: Abcam
• Catalog: ab20384
• Prices: $390.00
• Sizes: 200 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 8G1
• Applications: IHC-F IHC-P WB ICC/IF ICC/IF Electron microscopy DB FC
• Species Reactivities: Human
• Antigen: Full length native protein (purified) corresponding to LRP1 aa 1-172
• Description: Mouse Monoclonal
• Gene: LRP1
• Conjugate: Unconjugated

Product images

ab20384 staining human mesothelial cells by flow cytometry.  Cells were lifted in lifting buffer plus FBS and fixed with formaldehyde.  The cell population was gated against mIgG control.  The primary antibody was diluted 1/100 in 0.2% BSA in PBS and incubated with the sample at a concentration of 1 µg/100 µl for 1 hour at 4°C.  A FITC conjugated goat anti-mouse antibody was used as the secondary, diluted 1/100 and incubated with the sample at a concentration of 1 µg/100 µl.See Abreview

Ab20384 staining primary rat hepatocytes cells by EM. The cells were fixed and prepared by Tokuyasu method. The cryosections of 70nm were placed on 100 mesh grids. All the following steps were done at room temperature. Blocking was carried out for 30 min with 1% fish skin gelatine (FSG). The tissue sections were placed on a drop of 5 ul of first Ab (anti-LRP 8G1) for 20 min at dilutions of 1:400 or 1:600 seemed to work the best. After incubation with the primary antibody, 3. 5 x 2 min wash was done with PBS and the sections were placed on a drop of 5 ul of secondary Ab (goat anti-mouse) for 20 min (dilution 1:150 5. 5 x 2 min wash with PBS). Samples were placed on a drop of 5 ul of PAG10 for 15 min (dilution 1:60 or 1:70) and then 5 x 2 min wash with PBS. 2 min on 1% glutaraldehyde for fixation and 4 x 2.5 min on H2O. afterthat 1 second + 1 second + 6 min on drops of Methyl Cellulose/Uranyl Acetate (9:1) on ice. The surplus of MC/UA was removed , the slides were dried and observed at t

ICC/IF image of ab20384 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20384, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

High molecular mass assemblies of amyloid-beta oligomers bind prion protein in - High molecular mass assemblies of amyloid-beta oligomers bind prion protein in

Dohler F, Sepulveda-Falla D, Krasemann S, Altmeppen H, Schluter H, Hildebrand D, Zerr I, Matschke J, Glatzel M. Brain. 2014 Mar;137(Pt 3):873-86.

Differential regulation of extracellular tissue inhibitor of metalloproteinases-3 - Differential regulation of extracellular tissue inhibitor of metalloproteinases-3

Scilabra SD, Troeberg L, Yamamoto K, Emonard H, Thogersen I, Enghild JJ, Strickland DK, Nagase H. J Biol Chem. 2013 Jan 4;288(1):332-42.

A pilot study on low-density lipoprotein receptor-related protein-1 in Chinese - A pilot study on low-density lipoprotein receptor-related protein-1 in Chinese

Chan CY, Chan YC, Cheuk BL, Cheng SW. Eur J Vasc Endovasc Surg. 2013 Nov;46(5):549-56.

Sequence identity between the alpha 2-macroglobulin receptor and low density - Sequence identity between the alpha 2-macroglobulin receptor and low density

Strickland DK, Ashcom JD, Williams S, Burgess WH, Migliorini M, Argraves WS. J Biol Chem. 1990 Oct 15;265(29):17401-4.