Anti-LRP1 antibody [EPR3724]

• Supplier: Abcam
• Catalog: ab92544
• Prices: $404.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3724
• Applications: IHC-P WB IP FC ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Human, Pig
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human LRP1 aa 4450 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: LRP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/50000 dilution (purified)Lane 1 : A549 cell lysateLane 2 : Human fetal brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/50000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/50000 dilution (purified)Lane 1 : Pig liver tissue lysateLane 2 : Pig heart tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/20000 dilution (unpurified)Lane 1 : Human PMBC lysateLane 2 : A549 lysateLane 3 : Mouse brain lysateLane 4 : Mouse heart lysateLane 5 : Mouse kidney lysateLane 6 : Mouse spleen lysateLane 7 : Rat brain lysateLane 8 : Rat heart lysateLane 9 : Rat kidney lysateLane 10 : Rat spleen lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma of the kidney tissue labelling LRP1 with purified ab92544 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling LRP1 with unpurified ab92544.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human lung tissue labelling LRP1 with unpurified ab92544.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human placenta tissue labelling LRP1 with unpurified ab92544.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling LRP1 with unpurified ab92544 at 1/100.

Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling LRP1 with purified ab92544 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

ICC/IF image of LRP1 staining on rat mixed glia culture using unpurified ab92544 (1:200). The cells were fixed using paraformaldehyde. The cells were then permeabilised using 0.1% TritonX in 0.1% PBS. Non-specific protein was blocked using 10% donkey serum at 24°C for 1 hour. ab92544 was diluted (1/200) using 0.1% TritonX with 0.1% PBS and 10% donkey serum and the cells were incubated for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to Rabbit IgG conjugated to Alexa Fluor® 488. DAPI was used to stain the nucleus.See Abreview

Flow Cytometry analysis of Jurkat cells labelling LRP1 with purified ab92544 at 1/100 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Overlay histogram showing Jurkat cells stained with unpurified ab92544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92544, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ab92544 (purified) at 1/30 immunoprecipitating LRP1 in A549 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Product References

KIF13B enhances the endocytosis of LRP1 by recruiting LRP1 to caveolae. - KIF13B enhances the endocytosis of LRP1 by recruiting LRP1 to caveolae.

Kanai Y, Wang D, Hirokawa N. J Cell Biol. 2014 Feb 3;204(3):395-408.

Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin - Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin

Niesman IR, Schilling JM, Shapiro LA, Kellerhals SE, Bonds JA, Kleschevnikov AM, Cui W, Voong A, Krajewski S, Ali SS, Roth DM, Patel HH, Patel PM, Head BP. J Neuroinflammation. 2014 Mar 3;11:39.

LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a - LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a

Schorch B, Song S, van Diemen FR, Bock HH, May P, Herz J, Brummelkamp TR, Papatheodorou P, Aktories K. Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6431-6. doi:

The FAM3 superfamily member ILEI ameliorates Alzheimer's disease-like pathology - The FAM3 superfamily member ILEI ameliorates Alzheimer's disease-like pathology

Hasegawa H, Liu L, Tooyama I, Murayama S, Nishimura M. Nat Commun. 2014 Jun 4;5:3917.

Recurrent LRP1-SNRNP25 and KCNMB4-CCND3 fusion genes promote tumor cell motility - Recurrent LRP1-SNRNP25 and KCNMB4-CCND3 fusion genes promote tumor cell motility

Yang J, Annala M, Ji P, Wang G, Zheng H, Codgell D, Du X, Fang Z, Sun B, Nykter M, Chen K, Zhang W. J Hematol Oncol. 2014 Oct 10;7(1):76.

Aggregated low-density lipoprotein induces LRP1 stabilization through E3 - Aggregated low-density lipoprotein induces LRP1 stabilization through E3

Cal R, Garcia-Arguinzonis M, Revuelta-Lopez E, Castellano J, Padro T, Badimon L, Llorente-Cortes V. Arterioscler Thromb Vasc Biol. 2013 Feb;33(2):369-77. doi:

Cholesteryl esters accumulate in the heart in a porcine model of ischemia and - Cholesteryl esters accumulate in the heart in a porcine model of ischemia and

Drevinge C, Karlsson LO, Stahlman M, Larsson T, Perman Sundelin J, Grip L, Andersson L, Boren J, Levin MC. PLoS One. 2013 Apr 26;8(4):e61942.

Identification of the cellular sentinels for native immunogenic heat shock - Identification of the cellular sentinels for native immunogenic heat shock

Messmer MN, Pasmowitz J, Kropp LE, Watkins SC, Binder RJ. J Immunol. 2013 Oct 15;191(8):4456-65.

The hypolipidemic effect of cilostazol can be mediated by regulation of hepatic - The hypolipidemic effect of cilostazol can be mediated by regulation of hepatic

Kim HJ, Moon JH, Kim HM, Yun MR, Jeon BH, Lee B, Kang ES, Lee HC, Cha BS. Metabolism. 2014 Jan;63(1):112-9.

Insulin responsiveness of glucose transporter 4 in 3T3-L1 cells depends on the - Insulin responsiveness of glucose transporter 4 in 3T3-L1 cells depends on the

Huang G, Buckler-Pena D, Nauta T, Singh M, Asmar A, Shi J, Kim JY, Kandror KV. Mol Biol Cell. 2013 Oct;24(19):3115-22.